Simulation:DoseResponse
From QuB
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This function simulates the response of a Model to a series of pulses, then plots peak current vs. stimulus. To run this, you will need a model with at least one stimulus-dependent rate (a rate with P -- Ligand -- or Q -- Voltage -- selected).
If you give a list of pulse levels, QuB simulates a sweep of data at each level and measures the peak in each sweep.
To study cumulative or competitive effects, you can repeat each sweep a number of times. QuB then fits the sequence of peaks to an exponential and reports Tau.
Simulated data is shown in the Data window. Measurements are displayed in the Results window. You may need to View -> Results in order to see them.
Properties
| Sweep Duration | Length of one sweep, including resting and pulsing time, in milliseconds |
|---|---|
| Sampling | Sampling rate of the data to be simulated, in kHz |
| Repeat each sweep | Number of repeats per level listed on "Pulse levels" |
| Variable | Which "Experimental conditions" variable gets the pulse. You should already have activated this variable with the P or Q checkbox in Model Rate Properties. |
| Starts on repeat number | Optionally, you can have a variable not start pulsing, and stay at 0.0, for the first few reps |
| Resting level | Value for the stimulus variable, before and after the pulse |
| Pre-pulse duration | Time to rest before each pulse, in milliseconds |
| Pulse duration | Time to spend at pulse level each sweep, in milliseconds |
| Pulse levels | List of values for the stimulus variable; one per sweep, separated by comma or space |
| Pulse filter | Whether to low-pass filter the stimulus waveform |
| Time constant | Rise time for the stimulus filter, in milliseconds |
| Channel count | Number of ion channels to simulate; if this number is in the thousands, the simulation is deterministic |
| Filter cutoff | Whether to filter the simulated response data, and with what cutoff frequency (kHz) |
| Measure current at | peak: each sweep is measured at its maximum time: all sweeps are measured this many milliseconds from their start |
| Normalize current | Whether to also measure "Normalized Current", as (peak / max(all peaks)) |
| Save measurements | QuB can save the peak measurements as a tab-separated text file. |
See Also
| Prev: Simulation:StimSim | Outline | Next: Actions:Modeling |
